发明名称 |
Reverse two-hybrid systems |
摘要 |
Disclosed are methods for identifying molecular interactions (e.g., protein/protein, protein/DNA, protein/RNA, or RNA/RNA interactions). All of the methods within the invention employ counterselection and at least two hybrid molecules. Molecules which interact reconstitute a transcription factor and direct expression of a reporter gene, the expression of which is then assayed. Also disclosed are genetic constructs which are useful in practicing the methods of the invention. |
申请公布号 |
AU5541296(A) |
申请公布日期 |
1996.10.30 |
申请号 |
AU19960055412 |
申请日期 |
1996.04.11 |
申请人 |
THE GENERAL HOSPITAL CORPORATION;JOHNS HOPKINS UNIVERSITY., THE |
发明人 |
MARC VIDAL;JEF D. BOEKE;ED HARLOW |
分类号 |
C12N15/09;C12N1/19;C12N15/10;C12Q1/68;C12R1/865;G01N33/50;G01N33/53 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|