发明名称 |
Transgenic non-human animals capable of producing heterologous antibodies of various isotypes |
摘要 |
The invention relates to transgenic non-human animals capable of producing heterologous antibodies of multiple isotypes. Heterologous antibodies are encoded by immunoglobulin heavy chain genes not normally found in the genome of that species of non-human animal. In one aspect of the invention, one or more transgenes containing sequences that permit isotype switching of encoded unrearranged heterologous human immunoglobulin heavy chains are introduced into a non-human animal thereby forming a transgenic animal capable of producing antibodies of various isotypes encoded by human immunoglobulin genes. Such heterologous human antibodies are produced in B-cells which are thereafter immortalized, e.g., by fusing with an immortalizing cell line such as a myeloma or by manipulating such B-cells by other techniques to perpetuate a cell line capable of producing a monoclonal heterologous antibody. The invention also relates to heavy and light chain immunoglobulin transgenes for making such transgenic non-human animals as well as methods and vectors for disrupting endogenous immunoglobulin loci in the transgenic animal. |
申请公布号 |
US5569825(A) |
申请公布日期 |
1996.10.29 |
申请号 |
US19910810279 |
申请日期 |
1991.12.17 |
申请人 |
GENPHARM INTERNATIONAL |
发明人 |
LONBERG, NILS;KAY, ROBERT M. |
分类号 |
A01K67/027;A61K38/00;A61K39/395;C07K14/725;C07K16/00;C07K16/18;C07K16/28;C07K16/30;C07K16/42;C07K16/44;C07K16/46;C12N5/10;C12N5/16;C12N15/00;C12N15/09;C12N15/13;C12N15/85;C12N15/87;C12N15/90;C12P21/08;(IPC1-7):C12N15/00;C07H21/04 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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