| 摘要 |
PURPOSE: To produce the subject glucide useful as a synthetic intermediate, etc., for a complex glucide saccharide chain such as a glycoprotein or a glycolipid by carrying out the saccharide transfer reaction of a solution containing GlcNAc-Man and a galactose donor with a glycosidase. CONSTITUTION: An N-acetylhexosaminidase is reacted with a solution containing mannose (Man) and N-acetylglucosamine (GlcNAc) to prepare GlcNAc-Man. The resultant GlcNAc-Mann and a galactose donor (e.g. lactose) are then dissolved in a solution comprising dimethyl sulfoxide and 0.2M potassium phosphate buffer solution (pH 6.0) and a glycosidase (e.g.β-galactosidase) is added thereto. Reaction is carried out at 37 deg.C for 5hr under shaking and the reactional solution is then treated at 100 deg.C for 5min to inactivate the enzyme. The resultant solution is subsequently treated with an active carbon column, fractionated and purified at a linear concentration gradient of water-ethanol to afford the objective Gal- GlcNAc-Man useful as a synthetic intermediate, etc., for synthesizing a complex glucide saccharide chain such as a glycoprotein or a glycolipid.
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