| 摘要 |
<p>This invention relates to a method for sequencing nucleic acids, specifically sequencing DNA using primer extension reactions with deoxynucleoside triphosphates (dNTP's), dideoxynucleoside triphosphate terminators (ddNTP's), primers, and polymerase enzymes. The invention describes methods of generating slightly overlapping and rather narrow size-distribution subsets of sequencing fragments corresponding to contiguous regions along the sequence of the template nucleic acid. These subsets of overlapping contiguous sequencing fragments are rapidly separated in parallel on a set of electrophoresis runs, each of which is optimized to adequately resolve one of the distributions of sequencing fragments in minimal time. The subsets of nucleic acid sequence information generated from the set of electrophoretic separations allows reconstruction of the nucleic acid sequence of the template.</p> |
