发明名称 |
Recombinant production of chymase |
摘要 |
The instant invention is directed to recombinant production of functionally active chymase. "Functionally active" as used herein refers to the ability to exhibit one or more functional activities of a full-length wild-type chymase protein. In a preferred aspect, a proteolytically inactive chymase fusion protein comprising a functionally active portion of a non-chymase protein joined to the amino-terminus of the chymase protein is produced, which, upon cleavage away of the non-chymase fusion protein portion, becomes proteolytically active. A refolding procedure for increasing yields of proteolytically active recombinant chymase is provided. The invention is further directed to use of the recombinant chymase thus produced for preparing chymase-specific antibodies.
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申请公布号 |
US5567602(A) |
申请公布日期 |
1996.10.22 |
申请号 |
US19920929198 |
申请日期 |
1992.08.12 |
申请人 |
ARRIS PHARMACEUTICAL CORPORATION |
发明人 |
CLARK, JAMES M.;SHOEMAKER, KEVIN R.;WARNE, ROBERT L. |
分类号 |
C12N9/64;C12N15/57;C12N15/62;(IPC1-7):C12N15/57;C12N15/09;C12N15/10 |
主分类号 |
C12N9/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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