A reagent for analyzing cells in urine and a method for analyzing cells in urine with the use of said reagent are provided. The present invention discloses a reagent for analyzing cells in urine which comprises solution(s) containing a fluorescent dye, an osmolarity compensating agent and a buffer, as well as a method for analyzing cells in urine which comprises diluting a urine sample and staining cells therein with said reagent, irradiating said cells with light in the violet or blue wavelength region by using a flow cytometer and measuring the forward or side scattered light and fluorescence from said cells.