| 摘要 |
<p>A rapid automated method for identifying biologically active substances by their effect on living cells comprises the steps: a) culturing cells on/within a matrix that fluidically can be controlled; b) loading the cells with luminescent chemical molecules or having them previously transfected with recombinant molecules having luminescent properties; c) injecting a well defined volume of the cell carrying matrix suspension into a flow fluid which transports the suspension into a flow chamber having a wall through which the content of said flow chamber may be monitored; d) perifusing the cells with a sample containing a potentially biologically active substance; e) measuring the luminescence response of the content in the flow chamber; f) flushing the flow chamber to make it ready for a new testing cycle.</p> |
