发明名称 Method for molecular indexing categorising of expressed genes using restriction enzymes
摘要 <p>This invention relates to a method for classifying (indexing) cDNA which has been reverse-transcribed from tissue- or cell-derived RNA, or DNA in a short period without duplication by using class-IIS restriction enzymes or a combination of a class-IIS and a class-II restriction enzymes. According to this invention, it is possible to analyse and diagnose variations such as tumors easily, correctly and promptly by comparing the analyzed pattern of genes expressed in a cell or tissue sample with the analyzed pattern of normal genes. This method is also applicable to the search and isolation of genes of physiologically active substances that are potential pharmaceuticals or causative genes of hereditary diseases, as well as the isolation of those genes that are useful for improving agricultural products. &lt;IMAGE&gt;</p>
申请公布号 EP0735144(A1) 申请公布日期 1996.10.02
申请号 EP19960104817 申请日期 1996.03.26
申请人 RESEARCH DEVELOPMENT CORPORATION OF JAPAN 发明人 KATO, KIKUYA
分类号 C12N15/10;C12Q1/68;(IPC1-7):C12Q1/68;C07H21/04;C12P19/34 主分类号 C12N15/10
代理机构 代理人
主权项
地址