Methods and compositions for promoting protein folding

摘要

Disclosed are 1) compositions comprising isolated monomeric subunits of chaperonin-60 or truncated fragments thereof that promote the folding of a polypeptide chain in vitro, 2) monomeric subunits of chaperonin-60 or truncated fragments thereof, immobilized on a solid surface, that promote also the folding of a polypeptide chain in vitro, 3) methods for preparing and/or immobilizing the monomeric subunits of chaperonin-60 or truncated fragments thereof, and 4) methods for folding polypeptide chains, specifically polypeptide chains expressed in a heterologous expression system, in vitro using the monomeric subunits of chaperonin-60 or truncated fragments thereof.