发明名称 |
Process for selectively hydrolising fusion proteins |
摘要 |
<p>Polypeptides or proteins such as, for example, insulins or de-B<3><0>-insulins - especially human insulin or de-B<3><0>-human insulin - are prepared by enzymatic cleavage, using a trypsin-like endopeptidase, of specific fusion proteins which can be obtained by genetic manipulation and which contain the residue of a peptide-like alpha -amylase inhibitor in the molecule. The use of the specific fusion proteins results in a reaction product which can be worked up advantageously; the working-up can take place, where appropriate, by crystallisation.</p> |
申请公布号 |
GR3020297(T3) |
申请公布日期 |
1996.09.30 |
申请号 |
GR19960401606T |
申请日期 |
1996.06.20 |
申请人 |
HOECHST AKTIENGESELLSCHAFT |
发明人 |
VERTESY, LASZLO, DR.;SAUBER, KLAUS, DR.;EHLERS, EBERHARD, DR. |
分类号 |
C07K14/62;C12P21/06;(IPC1-7):C07K1/12;C12P21/02 |
主分类号 |
C07K14/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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