PROCEDE DE DETECTION DE MICROORGANISMES PAR SEPARATION ET CULTURE SUR UN SYSTEME GELIFIE, SYSTEME GELIFIE ET NECESSAIRE DE DOSAGE POUR METTRE EN OEUVRE CE PROCEDE, UTILISATION EN MICROBIOLOGIE

摘要

A method for detecting the presence or absence of microorganisms belonging to the group which consists of bacteria and yeasts in a liquid sample (5) of a biological material. The method comprises placing the liquid sample (5) in a centrifuge tube (6a, 6b) above a gelled system (10) comprising at least (a) a first so-called development phase (1), i.e. a gel comprising a microorganism culture medium and a reagent for inducing a detectable optical measurement change in the presence of microorganisms, said gel being an intimate mixture of water and water-absorbing polymeric particles that have swelled in such a way that, in said intimate mixture, said polymeric particles have ( alpha ) a dry weight concentration of 0.05-0.2 g/ml, and ( beta ) a swollen-state diameter of 90-320 mu m, the water in the intimate mixture being at least partially provided by said culture medium; centrifuging; and revealing the presence or absence of microorganisms in said liquid sample (5) at said first phase (1) of the gelled system (10) by means of said reagent inducing a detectable optical measurement change.