| 摘要 |
PURPOSE: To obtain the subject new safe protein having a specific amino acid sequence, originated from the smooth muscle of a rabbit stomach pylori part, having a G protein-conjugated receptor activity, and useful as a medicine for preventing and treating a G protein-conjugated receptor protein deficiency, etc. CONSTITUTION: A new G protein-conjugated receptor protein contains the same amino acid sequence as an amino acid sequence containing an amino acid sequence of the formula, is originated from the smooth muscle of a rabbit stomach pylori part, has a G protein-conjugated receptor activity, and is useful as an agent for clarifying the structure and property of the G protein-conjugated receptor, a medicine for preventing and treating a G protein-conjugated receptor protein deficiency, etc. The receptor protein is obtained by separating mRNA from the smooth muscle of the rabbit stomach pylori part by a conventional method, synthesizing a cDNA from the mRNA with negative transcriptase, cloning the synthesized cDNA as a template by a PCR method using a synthetic primer comprising a part of a gene, such as a man-originated galanin receptor, and expressing the obtained G protein-conjugated receptor gene in a host. |
