| 摘要 |
An immuno-chromatographic TSH assay has now been developed which produces a visible result within minutes of sample application. The assay comprises a labeled antibody in a mobile phase and a second antibody in a stationary phase. The antibodies are chosen such that they are preferably non-competitive; i.e., they bind to different epitopes on the TSH and thus form a "sandwich" complex when both antibodies are bound to the TSH. In operation, sample TSH reacts with the mobile antibody and then both are transported to the bound antibody and form a "sandwich" therewith. Over a short time, if there is sufficient TSH in the sample, the concentration of TSH increases until it produces a visible spot in the area of the stationary phase. The TSH assay of the present invention may be used in screening for primary hypothyroidism in neonates and adults. It may also be used in combination with TRH administration to screen for hyperthyroidism. |
