| 摘要 |
Method for obtaining oligonucleotides and sensors for the determination and identification of taxonomic groups, products obtained and applications. The method comprises: (a) selecting the strains, (b) extracting the DNA, (c) selecting oligonucleotides which are capable of hybridizing with one of the strands of the DNA and which have a free terminal 3'hydroxyl as starters, (d) performing the polymerase chain reaction, (e) analysing the DNA fragments which have been amplified, (f) selecting the bands common to all the organisms in the taxon from the AP- PCR standards, (g) sequencing the ends of the common AP-PCR fragments, (h) designing specific oligonucleotides which are complementary to the sequences obtained, (i) detecting the presence of specific fragments in the genome of an individual. The products obtained by this method have application in the determination and identification of taxonomic groups, e.g. bacteria, including specifically the species Haloferax mediterranei.
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