发明名称 |
IN VITRO-BEHANDLUNG VON FUSIONSPROTEINEN |
摘要 |
<p>There is provided a novel method for the production of a biologically active protein comprising treating a fusion protein consisting of 1. one or multiple successive protein segment(s) each consisting of said biologically active protein the C-terminal amino acid of which is joined to a linker polypeptide sequence L and 2. a polypeptide tag joined to the C-terminal amino acid of said successive protein segment(s), or consisting of 1. a polypeptide tag joined to the N-terminal amino acid of 2. multiple successive protein segments each consisting of a linker polypeptide sequence L and said biologically active protein, with soluble yeast endoprotease yscF and with soluble yeast carboxypeptidase ysc alpha , and isolating said biologically active protein.</p> |
申请公布号 |
AT141645(T) |
申请公布日期 |
1996.09.15 |
申请号 |
AT19910810543T |
申请日期 |
1991.07.09 |
申请人 |
CIBA-GEIGY AG |
发明人 |
HEIM, JUTTA, DR.;SEEBOTH, PETER, DR.;TAKABAYASHI, KENJI, DR. |
分类号 |
A61K38/00;A61K38/21;A61K38/22;A61K38/23;A61K38/28;A61K38/46;A61K38/55;A61K39/395;C07K14/005;C07K14/195;C07K14/435;C07K14/52;C07K14/555;C07K14/56;C07K14/575;C07K14/585;C07K14/65;C07K14/745;C07K14/815;C07K16/00;C07K19/00;C12N1/19;C12N1/21;C12N9/00;C12N9/60;C12N15/09;C12N15/62;C12P21/02;C12P21/06;C12R1/19;C12R1/865 |
主分类号 |
A61K38/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|