发明名称 |
METHOD OF PURIFICATION OF RECOMBINANT VIRAL VECTORS CONTAINING A THERAPEUTIC GENE |
摘要 |
The invention provides a method for purifying viral vectors containing therapeutic genes for use in gene therapy. The invention comprises a method of purification from a cell lysate of a recombinant viral vector containing a therapeutic gene, which comprises: a) treating said lysate with an enzymatic agent that selectively degrades both unencapsulated DNA and RNA; b) chromatographing the treated lysate from step a) on a first resin; and c) chromatographing the eluant from step b) on a second resin; wherein one resin is an anion exchange resin and the other is an immobilized metal ion chromatography (IMAC) resin.
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申请公布号 |
CA2214837(A1) |
申请公布日期 |
1996.09.12 |
申请号 |
CA19962214837 |
申请日期 |
1996.03.06 |
申请人 |
CANJI, INC. |
发明人 |
SHEPARD, H. MICHAEL;LIU, XIAODONG;SHABRAM, PAUL W.;HUYGHE, BERNARD G. |
分类号 |
C12N7/02;C12N15/86;(IPC1-7):C12N15/86 |
主分类号 |
C12N7/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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