SIMPLIFIED PROCEDURE FOR THE ISOLATION OF ANALYTICALLY PURE VICINE [2,6-DIAMINO-4,5- DIHYDROXYPRYRIMIDINE 5-(.beta.-D GLYCOPYRANOSIDE)] AND CONVICINE [2,4,5-TRIHYDROXY-6-AMINOPYRIMIDINE 5- (.beta.-D GLYCOPYRANOSIDE)]

摘要

A SIMPLIFIED PROCEDURE FOR THE ISOLATION OF ANALYTICALLY PURE VICINE ¢2,6-diamino-4,5-dihydroxypyrimidine 5-(B-D glucopyranoside)! AND CONVICINE ¢2,4,5-trihydroxy-6-amino-pyrimidine 5-(B-D glycopyranoside)! Conventional methods of preparing vicine and convicine utilize relatively slow, expensive and inefficient procedures and considerable difficulty is encountered in obtaining pure concentrations of either chemical. The present method overcomes disadvantages inherent in conventional procedures and produces an exceptionally high quality product. A crude extract of vincine and convicine is first prepared by using a protein-rich concentration of faba beans or the like which is extracted with a basic aqueous-organic solvent which is then concentrated by evaporation. The pH is then adjusted so that crystallization of the vicine and convicine takes place and the crystals are harvested by centrifugation or the like thus producing a crude vicine-convicine isolate. The vicine can then be extracted from the isolate by adjustment of pH due to the fact that the pH has a differential effect on the solubility of the vicine and convicine.