发明名称 |
Method of measuring oligonucleotide decomposition |
摘要 |
<p>The present invention provides an in situ monitoring of the decomposing activity against oligonucleotides target in a biological tissue. A single-chain oligonucleotide target comprises an appropriate number of nucleic acid bases to be examined with an energy donor and an energy acceptor respectively at its 5'- and 3'-terminals. Monitoring of the fluorescence changes of the target after injection into a biological tissue, particularly the fluorescent resonance energy transfer(FRET) phenomena between the energy donor and acceptor, indicates whether the oligonucleotide is not decomposed yet. <IMAGE></p> |
申请公布号 |
EP0731178(A2) |
申请公布日期 |
1996.09.11 |
申请号 |
EP19960301597 |
申请日期 |
1996.03.08 |
申请人 |
HAMAMATSU PHOTONICS K.K. |
发明人 |
UCHIYAMA, HISATOSHI;JIBU, MASAKI;HIRANO, KENICHI;TAIRA, KAZUNARI |
分类号 |
C07H21/00;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C07H21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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