Process for the synthesis of ribonucleic acid (RNA) using a novel deprotection reagent

摘要

PCT No. PCT/FR92/00929 Sec. 371 Date Jul. 26, 1994 Sec. 102(e) Date Jul. 26, 1994 PCT Filed Oct. 7, 1992 PCT Pub. No. WO93/07164 PCT Pub. Date Apr. 15, 1993Process for RNA synthesis consisting in successively condensing necleosides for the formula (1) in which R1 is a puric pyrimidic optionally protected base, R2 is a leaving group such as the dimethoxytrityl radical, R3 is a phosphorus-containing radical, for example, phosphoramidite and R4 is a trialkylsilyl radical. The RNA obtained is then subjected to a protective treatment with a reagent of the formula (II), for example N(C2H5)3,3HF, to eliminate R4 protective groups of the 2' hydroxyl groups. Such reagent provides full deprotection in about ten hours, with an RNA of great length, for example, having more than 50 bases.