The invention provides a method of screening a substance for the ability to effect the formation of a retinoid X receptor homodimer comprising combining the substance and a solution containing retinoid X receptors and determining the presence of homodimer formation. Also provided is a method of screening a substance for an effect on a retinoid X receptor homodimer's ability to bind DNA comprising combining the substance with the homodimer and determining the effect of the compound on the homodimer's ability to bind DNA. A method of inhibiting an activity of a retinoid X receptor heterodimer comprising increasing the formation of a retinoid X receptor homodimer, thereby preventing the retinoid X receptor from forming a heterodimer and preventing the resulting heterodimer activity is also provided. A method of inhibiting an activity of a retinoid X receptor homodimer is also provided. In addition, a method of screening a response element for binding with a retinoid X receptor homodimer is provided. Finally, the invention provides methods of activating retinoid X receptor homodimer formation.
申请公布号
US5552271(A)
申请公布日期
1996.09.03
申请号
US19920982174
申请日期
1992.11.25
申请人
LA JOLLA CANCER RESEARCH FOUNDATION
发明人
PFAHL, MAGNUS;ZHANG, XIAO-KUN;LEHMANN, J+E,UML U+EE RGEN M.;DAWSON, MARCIA I.;CAMERON, JAMES F.;HOBBS, PETER D.;JONG, LING