HIGH SENSITIVITY DETECTION OF PEROXIDASE ACTIVITY

摘要

P-1356 HIGH SENSITIVITY DETECTION OF PEROXIDASE ACTIVITY A new class of peroxidase substrates consists of o-diaminobenzenes having a substituent in the position. The new substrates may be used in immunoassays in which a peroxidase is the lable, or in assays for the peroxidase itself. Immunoassays using other enzyme labels which cause formation of hydrogen peroxide may be followed using the substrate of the invention by adding the substrate and a peroxidase to the assay fluid and detecting color formed as a result of oxidation of the substrate by the formed peroxide.