摘要 |
<p>The invention relates to a method and culture medium for in vitro culturing eucaryotic cells requiring glutamine, which comprises the use of a protein hydrolysate obtained by enzymatic hydrolysis of a protein material. The protein hydrolysate has a free amino acid level of less than 15 % by weight and the protein hydrolisate have molecular weight below 44 kD. The protein hydrolysate preferably contains at least 20 % of glutamine residues. Also, preferably at least 90 % by weight of the peptides have a molecular weight below 1000 D and the average peptide chain length preferably is below 15 amino acid residues.</p> |