发明名称 |
CARBOHYDRATE-DEFICIENT TRANSFERRIN ASSAY |
摘要 |
The invention provides a method of assessment of carbohydrate-deficient transferrin in a transferrin containing body fluid, said method comprising the steps of: i) obtaining a transferrin containing liquid sample of or derived from a said fluid; ii) contacting said sample with a source of iron ions; iii) subsequently contacting said sample with an anionic ion exchange resin at a pH such as to cause carbohydrate-deficient transferrin to be retained by said resin; iv) subsequently contacting said resin with an eluant serving to release carbohydrate-deficient transferrin into the eluate from said resin; v) collecting a volume of said eluate substantially free from tetra- and penta-sialo transferrin; and vi) assessing the transferrin variant content in said volume of eluate. By including at least a proportion of the trisialotransferrin in the eluate, it is possible to use relatively simple assessment techniques such as turbidimetry in the assay. |
申请公布号 |
WO9626444(A1) |
申请公布日期 |
1996.08.29 |
申请号 |
WO1996GB00395 |
申请日期 |
1996.02.21 |
申请人 |
AXIS BIOCHEMICALS A/S;COCKBAIN, JULIAN;SUNDREHAGEN, ERLING |
发明人 |
SUNDREHAGEN, ERLING |
分类号 |
G01N33/48;G01N33/53;G01N33/68;G01N33/84;G01N33/98;(IPC1-7):G01N33/68 |
主分类号 |
G01N33/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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