| 摘要 |
PURPOSE: To obtain the subject new antibody comprising light chain and heavy chain variable region of mouse monoclonal antibody against human interleukin-8 (IL-8) and human light chain and heavy chain stationary region and low in antigenicity to human and useful as medical therapy use, etc. CONSTITUTION: This reconstituted human antibody is obtained by fusing a spleen cell collected by immunizing human IL-8 into a mouse with a mouse myeloma cell, cloning the fused cell to prepare hybridoma, separating mRNA from the resultant hybridoma, using the hybridoma as a template to synthesize cDNA, cloning the cDNA with a primer to afford DNA coding a mouse monoclonal antibody light chain (L chain) variable region (V region) and a heavy chain (H chain) V region, each binding the mouse L chain V region DNA as well as human L chain stationary region (C region) DNA and human L chain V region framework region (FR) DNA, and the mouse H chain V region DNA as well as the human H chain C region DNA and the human H chain V region FR DNA to expression vectors and culturing a host cell transformed with these both expression vectors. |
