Method for conversion of a starch material, and enzyme composition suitable therefor

摘要

Conversion of a starch material utilizing a thermostable non-reducing glucan-forming enzyme activity (NRGFE), for instance from Sulfolobus solfataricus. Native starch is depolymerized at 60-120 DEG C and pH 4-9 in the presence of a thermostable NRGFE by means of thermostable glucanases, such as alpha -amylases, isoamylases, pullulanases and/or amylopullulanases, with formation of Maillard products having been suppressed by virtue of the NRGFE activity present. The non-reducing glucans obtained can be used for the formation of trehalose by treating them with glucanases such as amylases, glucosidases and/or amyloglucosidases. Also, they can be modified with the aid of glucosyltransferases, for instance be treated with a thermostable phosphorylase in the presence of a glucosyl donor to raise the degree of polymerization, or be derivatized chemically or otherwise, optionally followed by a new hydrolytic treatment.