发明名称 |
DIOXETANE COMPOUNDS FOR THE CHEMILUMINESCENT DETECTION OF PROTEASES, METHODS OF USE AND KITS THEREFORE |
摘要 |
1,2-Dioxetane compounds bearing a proteolytic enzyme specific amino acid or peptide are provided, which amino acid or peptide can be removed by action of the corresponding protease. When the amino acid or peptide is removed, the 1,2dioxetane decomposes with chemiluminescence, the generation of light providing a rapid, ultra-sensitive and convenient means for detecting the presence of the protease in the sample being inspected. The amount of light generated, or degree of chemiluminescence, can be correlated with the amount of protease present. Immunoassays, as well as DNA hybridization and DNA probe assays are provided.
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申请公布号 |
CA2212669(A1) |
申请公布日期 |
1996.08.15 |
申请号 |
CA19962212669 |
申请日期 |
1996.01.22 |
申请人 |
TROPIX, INC. |
发明人 |
BRONSTEIN, IRENA;VOYTA, JOHN C.;SPARKS, ALISON;EDWARDS, BROOKS;MARTIN, CHRISTOPHER |
分类号 |
G01N33/573;C07D321/00;C07K5/072;C07K5/08;C07K5/09;C07K5/10;C12Q1/37;G01N33/58;(IPC1-7):C07K2/00;C07F7/10;C07K1/13;C07F9/655;C12Q1/68 |
主分类号 |
G01N33/573 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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