| 摘要 |
<p>Assay protocols are provided for determining the interrelationship between lesion formation and cellular repair mechanisms of such lesions. The assay provides for synthesis of duplex oligomers, which may then be subjected to agents, which result in lesion formation. The modified DNA oligomers are desirably bound to a solid support and contacted with cell lysates and the ability of the cell lysate to correct lesion determined. The assay may be employed in various diagnostic contexts, such as evaluating response of neoplastic cells to therapeutic treatments, the effect of environments on causing lesions, and the ability of disease cells to repair various lesions, particularly those associated with the disease.</p> |
