| 摘要 |
PURPOSE: To obtain an ultra-short-chain miniribozyme capable of specifically cutting a RNA while maintaining ribozyme activity, and useful as an active ingredient for agents for medical, animal or agricultural use. CONSTITUTION: This miniribozyme contains a sequence of the formula (X is a mononucleotide selected from sequences complementary with that of a target RNA, being A, G, C, U, d, A, dG, dC or dT; (m) is an integer of >=2; (n) is an integer of >=3; Y is A, G, C or U), e.g. CGGGGCUGAUGAGCGAAACGGC. This miniribozyme can be synthesized by the β-cyanoethyl phosphoamidite method. |
