METHOD OF EXPANDING AND TRANSPLANTATION OF HEMATOPOIETIC CELL

摘要

The application concerns a method for the ex vivo expansion of hematopoietic cells, including hematopoietic stem and progenitor cells, using endothelial support cells in an artificial capillary system (ACS). The invention also concerns improved bone marrow transplantation methods. Also, the application concerns a method for enhancing the ex vivo expansion of hematopoietic cells by coating the capillaries of an ACS with a suitable coating reagent. A method for transducing ex vivo expanding hematopoietic cells, including hematopoietic stem and progenitor cells, with packaged recombinant retrovirus vectors is also provided.