发明名称 |
HIGH EFFICIENCY TRANSLATION OF MRNA MOLECULES |
摘要 |
An increased level of translation of a selected mRNA molecule is effected by coupling specific nucleotide sequences at the 5'- and 3' -ends of a nucleic acid molecule transcribable to or which itself is the mRNA molecule. The nucleotide sequence at the 5'-end is effective to increase the rate of translation initiation of the mRNA molecule in a cell while the nucleotide sequence at the 3'-end is effective to increase the period of translation of the mRNA molecule in a cell. The nucleotide sequence of the 3'-end is provided by a 3'-untranslated region (3'-UTR) of a gene, particularly that of .beta.-prolactin, or an effective fragment thereof. A polyadenylation sequence preferably is provided at the 3'-end of the 3'-UTR sequence. The 3'-UTR sequence provides mRNA stabilization independent of the poly A tail.
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申请公布号 |
CA2169317(A1) |
申请公布日期 |
1996.08.11 |
申请号 |
CA19962169317 |
申请日期 |
1996.02.12 |
申请人 |
MCMASTER UNIVERSITY |
发明人 |
ANDREWS, DAVID W.;HUGHES, MARTIN JOHN GLENTON;VASSILAKOS, AKATERINI |
分类号 |
C12N15/67;(IPC1-7):C12N15/67 |
主分类号 |
C12N15/67 |
代理机构 |
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地址 |
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