ENRICHMENT OF HEMATOPOIETIC STEM CELLS FROM BLOOD OR BONE MARROW

摘要

Process for enrichment of hematopoietic stem cells in a stem cell preparation, e.g. bone marrow, umbilical cord blood and peripheral blood stem cell preparation. Non-stem cells, in particular monocytes and myeloid cells, are depleted from the preparation by adherence to an opsonized material, e.g. a scrubbed nylon fiber coated with human IgG and blocked with human serum albumin. Platelets are depleted from the stem cell preparation before contacting the opsonized material, e.g. by counterflow centrifugal elutriation. Alternatively, adherence of platelets to stem cells is prevented by the addition of a chelating substance to the medium in which the enrichment process is performed.