| 摘要 |
PURPOSE: To efficiently obtain long-chain fatty acids which are frequently utilized as a starting substance for oleochemicals in industry by culturing a microorganism, Francisella sp. WYSA-40-2 and collecting the product from the culture mixture. CONSTITUTION: Francisella sp. strain: WYSA-40-2 (FERM P-14621) is inoculated to the culture medium, to which calcium carbonate or the like is added to keep the pH at 6-8 and shaking culture is continued at 20 deg.C for 2 days to produce and accumulate long-chain fatty acids in cell bodies. When the products reach the maximum in the culture mixture, the mixture is centrifuged to separate the cell bodies, which are freeze-dried, crushed, extracted with chloroform according to the Bligh & Dyer method. The solvent is distilled off from the extract under reduced pressure and the residue is purified to give long-chain fatty acids of high purity, useful as a starting substance for oleochemicals in industry, efficiently. |
