| 摘要 |
PURPOSE: To obtain the subject gene capable of increasing the sucrose-utilizing ability of a Coryneform bacterium and useful for obtaining an L-amino acid or nucleic acid in a good yield in a short time from raw materials containing the sucrose. CONSTITUTION: This gene is originated from a Coryneform bacterium and codes a protein which has the amino acid sequence of the formula and which has a sucrase activity. The gene is obtained by a method described in Japanese unexamined patent HEI 5-244958, comprising producing a gene library from the chromosome DNA of the Coryneform bacterium, subjecting the gene library to a colony hybridization with a probe synthesized under the homology in the base sequence of the sucrase gene, selecting a sucrase activity-expressing colony as a sucrase gene-introducing strain from hybridized colonies, and subsequently obtaining a DNA fragment containing the sucrase gene from the cell of the strain. |
