| 摘要 |
PURPOSE: To obtain a DNA sequence useful for obtaining an enzyme related to the fertilization, development, differentiation, canceration, etc., of organisms and useful for diagnostic medicines for various diseases, reagents for the researches of cytobiolory, etc. CONSTITUTION: This DNA sequence contains a structural gene coding a protein related to β-1,4-galactosyl transferase, can express the protein in a microorganism or an animal cell, and has e.g. an amino acid sequence of the formula. β-1,4-galactosyl transferase isolated from F9EC cells and subsequently purified is administered as an antigen into a rabbit. A serum is collected from the rabbit and subsequently blunted on a 1,4-galactosyl transferase protein-immobilized affinity column to produce an antibody. A positive colony is obtained from a λ GT 11 expression library prepared from the F9EC cells by the immunity- specific selection of colonies with the antibody, and the sequence of the cDNA insertion part of the positive colony is determined by Sanger method to obtain the cDNA. |
