发明名称 |
Method and apparatus for covalent immobilization of charge- conjugated carbohydrate molecules |
摘要 |
A method for covalent immobilization of a carbohydrate molecule with an oppositely charged surface, comprising the steps of adsorbing the carbohydrate molecule to the oppositely charged surface in proximity to reactive moieties bound to the charged surface; and next activating the bound moiety sufficiently for covalent attachment to the adsorbed carbohydrate molecule. Also disclosed is a hydrophobic microporous polymer membrane coated with a cross-linked, cationic polymer having fixed charges thereon, the coating having enhanced epoxide content for covalent immobilization of the oppositely-charged carbohydrate molecule. A novel method for sequencing carbohydrates by covalent attachment of a carbohydrate conjugate to the membrane, and subsequent treatment with glycosidases, is also presented.
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申请公布号 |
US5543054(A) |
申请公布日期 |
1996.08.06 |
申请号 |
US19930157805 |
申请日期 |
1993.11.24 |
申请人 |
MILLIPORE CORPORATION |
发明人 |
CHARKOUDIAN, JOHN;PLUSKAL, MALCOLM;WANG, DAVID;PHOEBE, CHARLES |
分类号 |
B01D67/00;G01N27/447;G01N33/543;G01N33/545;(IPC1-7):B01D61/38;C07H1/06;G01N27/26 |
主分类号 |
B01D67/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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