Monoclonal and polyclonal antibodies and test method for determination of organophosphates

摘要

Monoclonal and polyclonal antibodies and fragments thereof are described which are capable of binding to specific members or groups of O,O-dialkyl-O-(X) phosphorothioate compounds, where X is an aromatic or heterocyclic group or substituted aromatic or substituted heterocyclic group. Specifically, organophosphorus pesticides (e.g. fenitrothion, chloryrifos-methyl, and pirimiphos-methyl) are conjugated to a carrier with a beta-alanine spacer and used as an immunogen for raising antibodies. Hybridoma cell lines, methods for making the antibodies, and assays and kits including the antibodies are also disclosed.