| 摘要 |
PURPOSE: To obtain the subject derivative for biological functional materials, etc., by amide bonding ofα-amino group of L-lysin residue of a part or all of a poly(ε-L-lysin) with D-galactopyranosylgluconic acid, having good cell adhesion property and cell proliferation property. CONSTITUTION: This D-galactopyranosylgluconic acid derivative of a poly-αsubstitutedε-L-lysin is obtained by dissolving a poly(ε-L-lysin) in 50mM N,N,N', N'-tetramethylethylenediamine(TEMED) buffer solution, adding D- galactopyranosylgluconic acid and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) thereto, reacting these components for three whole days and nights at room temperature, transferring the reaction solution to a dialyzing tube and carrying out dialysis to distilled water and lyophilizing the dialyzed material and subjectingα-amino group of L-lysin residue in a part or all of L-lysin residue of a poly(ε-L-lysin) expressed by the formula [(n) and (m) are integer of >=1; (n)>=(M)] to amide bonding with D-galactopyranosyl gluconic acid.
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