| 摘要 |
<p>A method for preparing test articles comprises immobilizing a hapten-carrier molecule conjugate on a solid phase surface. Attachment of a hapten is effected through the carrier molecule which non-covalently adsorbs to the surface. The hapten is a small molecule which retains binding activity even under relatively harsh conditions which would degrade more labile substances such as antibodies and other binding proteins. Such labile binding proteins may subsequently be introduced to the solid phase through binding with anti-hapten binding substance under mild conditions. Optionally, the carrier molecule can be covalently attached to the surface after initial non-covalent position, for example by radiation-induced cross-linking.</p> |
