| 摘要 |
The present invention relates to ratchet libraries composed of related peptides synthesized simultaneously in a single peptide synthesis. Ratchet libraries are derived from a longer template peptide by sequentially "ratcheting" the template sequence into the shorter ratchet length and are used for cytotoxic T lymphocyte (CTL) induction or stimulation if the CTL epitope is known. If the CTL epitope is unknown, then the ratchet library can be used for identification of CTL epitopes. The ratchet libraries can be prepared from any protein sequence to which an immune CTL response is desired and can be formulated for delivery as a vaccine or therapeutic for the treatment or prevention of disease or malignancy. For example, a ratchet library can be used in the prevention and treatment of infectious or malignat diseases including HIV, influenza, malaria, breast, ovarian, lung and colon cancers. |
