| 摘要 |
PURPOSE: To efficiently obtain the objective protein by transforming a host cell with an expression vector containing a gene of a fused protein comprising a protective polypeptide and an object polypeptide, manifesting the gene and cutting with an endogenous protease of the host. CONSTITUTION: A host cell (e.g.; Escherichia coli) is transformed by an expression vector integrated a gene coding fused protein expressed by the formula: A-L-B (A is a protective polypeptide; B is the object polypeptide; L is a linker peptide having a substrate specifically recognized by an endogenous protease of a host cell) or the formula: A-L-B-L-C (C is a protective peptide) and comprising a protective polypeptide and the object polypeptide, cultured and a gene is manifested, the cell is crushed and the fused protein is separated, then the fused protein is solubilized and the linker peptide is cut with the endogenous protease of the host cell (e.g.; Escherichia coli ompT protease), thus the object polypeptide is efficiently obtained from the fused protein. |
