发明名称 |
Bacteriophage resistant recombinant bacteria |
摘要 |
Recombinant bacteria containing phage-encoded resistance ("Per") and methods of making and using the same are disclosed. Such bacteria are made by (a) conducting a fermentation of a substrate in a medium containing a defined bacterial culture until bacteriophage are detected in the medium, the bacteriophage being specific to at least one bacteria in the medium; (b) isolating the bacteriophage; (c) digesting DNA of the bacteriophage to produce a library of DNA fragments; (d) transforming the bacteria susceptible to said bacteriophage with the library of DNA fragments to provide transformed bacteria; (e) selecting from among the transformed bacteria, a bacteriophage-resistant transformed bacteria; (f) adding bacteriophage resistant transformed bacteria to the medium; and (g) recommencing step (a). Also disclosed are bacterial cells which contain a first bacteriophage defense mechanism (Per), wherein Per comprises a bacteriophage origin of replication (ori) operatively associated with a DNA sequence incapable of producing live bacteriophage. The bacterial cell is capable of being infected by a bacteriophage, the DNA of which, once injected into the bacterial cell, competes with Per for binding to DNA polymerase.
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申请公布号 |
US5538864(A) |
申请公布日期 |
1996.07.23 |
申请号 |
US19950373877 |
申请日期 |
1995.01.17 |
申请人 |
NORTH CAROLINA STATE UNIVERSITY |
发明人 |
HILL, COLIN J.;KLAENHAMMER, TODD R. |
分类号 |
A23C9/123;C12N1/04;C12N1/21;C12N15/11;C12N15/34;C12N15/65;C12N15/74;(IPC1-7):C12N1/20;A23C9/00;C12P7/56 |
主分类号 |
A23C9/123 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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