发明名称 |
Variant-type carbohydrate hydrolase, variant gene of the enzyme and method for producing oligosaccharide using the enzyme |
摘要 |
There is disclosed a variant-type carbohydrate hydrolase that has been increased transglycosylation activity by substituting another amino acid residue for the tyrosine residue that is present in the active center of the hydrolase, which hydrolase is an amylase or an enzyme analogous to amylase; a gene or a DNA sequence of the carbohydrate hydrolase with mutation introduced into the base sequence that encodes the tyrosine residue; and a vector or a transformant which comprises the DNA sequence. There is also disclosed a method for producing a variety of oligosaccharides and the like by using the variant-type carbohydrate hydrolase.
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申请公布号 |
US5538882(A) |
申请公布日期 |
1996.07.23 |
申请号 |
US19940204656 |
申请日期 |
1994.03.02 |
申请人 |
DIRECTOR-GENERAL OF AGENCY OF INDUSTRIAL SCIENCE AND TECHOLOGY |
发明人 |
MATSUI, IKUO;ISHIKAWA, KAZUHIKO;MIYAIRI, SACHIO;HONDA, KOICHI |
分类号 |
C12N9/10;C12N9/30;C12N15/09;C12N15/54;C12P19/14;C12P19/18;C12R1/07;C12R1/125;C12R1/645;C12R1/865;(IPC1-7):C12N9/10;C12N15/56 |
主分类号 |
C12N9/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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