发明名称 |
Nonhuman model animal of Th2-mediated hyperimmune response |
摘要 |
An object of the present invention is to provide a nonhuman model animal of Th2-mediated hyperimmune response lacking PIR-B gene function on its chromosome by which the Th2-mediated immune response mechanism and allergy onset mechanism in vivo can be analyzed and which is liable to suffer from not only hyper-response of B cells but also allergy, and an inducer/promoter or an inhibitor for Th2-mediated immune response, etc. with the use of the nonhuman model animal of Th2-mediated hyperimmune response. The nonhuman model animal of Th2-mediated hyperimmune response is prepared by integrating a fragment comprising exons 1 to 7 and the domain in the 5' side of exon 8 of mouse PIR-B gene and another fragment containing exons 10 to 14 into a vector pMC1-Neo, cleaving it with Xho I-Sal I, integrating it into a vector pIC19R-MC1tk having herpes virus thymidine kinase to thereby construct targeting vector, transferring the targeting vector into ES cells and then injecting the ES cells into blastcyst.
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申请公布号 |
US7301068(B2) |
申请公布日期 |
2007.11.27 |
申请号 |
US20040493835 |
申请日期 |
2004.11.30 |
申请人 |
JAPAN SCIENCE AND TECHNOLOGY CORPORATION |
发明人 |
TAKAI TOSHIYUKI;UJIKE-ASAI AZUSA |
分类号 |
A01K67/027;A01K67/033;A61K45/00;A61P37/08;C07K14/705;C12N15/09;C12N15/85;C12Q1/02;G01N33/00;G01N33/15;G01N33/50 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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