发明名称 |
RETINOID X RECEPTOR-INTERACTING POLYPEPTIDES AND RELATED MOLECULES AND METHODS |
摘要 |
Disclosed is a method for determining whether a test protein is capable of interacting with a retinoid X receptor protein. The method involves: (a) providing a host cell which contains (i) a reporter gene operably linked to a protein binding site; (ii) a first fusion gene which expresses a first fusion protein, the first fusion protein including a retinoid X receptor protein covalently bonded to a binding moiety which is capable of specifically binding to the protein binding site; and (iii) a second fusion gene which expresses a second fusion protein, the second fusion protein including the test protein covalently bonded to a gene activating moiety; and (b) determining whether the test protein increases expression of the reporter gene as an indication of its ability to interact with the retinoid X receptor protein. Also disclosed is purified DNA encoding retinoid X receptor-interacting proteins and the polypeptides expressed from such DNA.
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申请公布号 |
WO9621677(A1) |
申请公布日期 |
1996.07.18 |
申请号 |
WO1995US16311 |
申请日期 |
1995.12.08 |
申请人 |
THE GENERAL HOSPITAL CORPORATION |
发明人 |
MOORE, DAVID;SEOL, WONGI;CHOI, HUENG-SIK, |
分类号 |
G01N33/566;C07K14/47;C07K14/705;C12N5/10;C12N15/09;C12P21/02;C12Q1/68;C12R1/91;(IPC1-7):C07K14/705;C07H21/04;C12N1/21;C12N5/16;C12N15/12;C12N15/63;C12P21/00;C12Q1/25;G01N33/53 |
主分类号 |
G01N33/566 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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