| 摘要 |
<p>The present invention addresses compositions and methods for cleaving nucleic acids. The invention allows one to reliably detect point mutations in long and short target regions of nucleic acids in a safe, non-labor intensive, and cost effective manner. The methods and compositions of the present invention allow for the use of various RNases, such as RNase A, RNase I and RNase T1, in the detection of mutations. The present invention also identifies reaction conditions that result in significant improvement in specific mismatch cleavage in the NIRCATM assay.</p> |
