发明名称 |
PURIFIED THROMBOPOIETIN AND METHOD OF MAKING IT |
摘要 |
Purified mammalian thrombopoietin proteins and methods of making them are disclosed. The proteins are characterized by a Mr=70,000 +/- 10,000 daltons as determined by SDS-polyacrylamide gel electrophoresis under denaturing conditions and are at least 90 % pure with respect to contaminating proteins. The proteins can be prepared by a method in which thrombopoietin is adsorbed to and eluted from a polypeptide comprising a ligand-binding domain of an MPL receptor, then the eluted thrombopoieting is fractionated by anion exchange chromatography. |
申请公布号 |
WO9620955(A1) |
申请公布日期 |
1996.07.11 |
申请号 |
WO1995US16626 |
申请日期 |
1995.12.20 |
申请人 |
ZYMOGENETICS, INC. |
发明人 |
FORSTROM, JOHN, W.;LOFTON-DAY, CATHERINE, E.;LOK, SI |
分类号 |
C12N15/09;A61K38/00;A61P7/06;C07K1/18;C07K1/22;C07K1/26;C07K7/06;C07K14/52;C07K14/715;C12P21/02;C12R1/91 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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