RAPID ASSAY OF ACTIVATORS AND INHIBITORS OF CLOTTING

摘要

Assays and reagents are provided for the measurement of components that are involved in either enzyme-catalyzed degradation of a substrate, such as in fibrinogenolysis or fibrinolysis; or enzyme-catalyzed polymerization of a substrate, such as in fibrinogen polymerization into fibrin. The method involves using a fluorescent-labeled substrate in an enzyme-catalyzed reaction, and measurement of the component's effects on the fluorescent emission of the enzyme-catalyzed reaction such as quenching or dequenching, as a measure of the component's activity.