| 摘要 |
The invention relates to a method of detecting, identifying and quantitating microorganisms, and to oligonucleotide probes for use in this method. In particular, the method relates to the typing of specific isolates of microorganisms, and discrimination between strains and allelic subtypes. The method uses amplification of the 16S-23S rRNA spacer region using a highly conserved region from the 3' end of the 16S-23S rRNA spacer region, and/or a highly conserved region from the 5' end of said region. The method enables epidemiological tracing of specific microorganism subtypes. Preferred primers are disclosed and claimed. |
