发明名称 METHOD FOR PRODUCING WAVE FORM SIGNAL FOR DETERMINING BASE SEQUENCE AND DEVICE FOR DETERMINING BASE SEQUENCE
摘要 PURPOSE: To produce a wave form signal for determining a base sequence wherein ghost signal components are removed from the wave form signal of fluorescent strength per time, by multiplying a ghost generation rate by the receiving strengths of the ghost signal components from remaining three signal components in four nucleic acid bases. CONSTITUTION: In a DNA sequencer 1, the fluorescent light, etc., of a base fragment group having a prescribed width and emitted from a migration plate 2 irradiated with a laser light L is received on a CCD 3 through a lens, and the receiving strengths of the fluorescence, etc., in each lane in which four kinds of DNA bases are migrated in the presence of a color pigment marker is detected as analogous signals. The detected analogous signals are outputted to a computing treatment device 5 through a CCD driving/controlling circuit 4. In the computing treatment device, ghost signal components due to three bases except the target base component are reduced to produce a wave form signal. The wave form signals of the remaining three bases are multiplied by a ghost generation rateε, and the obtained ghost component is removed to produce the wave form signal. The same treatments are performed also for RNA.
申请公布号 JPH08173196(A) 申请公布日期 1996.07.09
申请号 JP19940338516 申请日期 1994.12.28
申请人 HITACHI ELECTRON ENG CO LTD 发明人 USUI SANPEI;SAKURAI TOSHIYUKI;TAKATSUKI NOBORU
分类号 G01N33/50;C12M1/00;C12Q1/68;G01N21/17;G06F17/30;(IPC1-7):C12Q1/68 主分类号 G01N33/50
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