| 摘要 |
PURPOSE: To carry out the large-scale artificial culture of fruit body of Cordyceps sinensis Sacc. which is equivalent to natural fruit body by culturing Cordyceps sinensis Sacc. in sterilized dried chrysalis to form a mycelium, culturing the mycelium in soil and germinating fruit body thereof on the soil surface. CONSTITUTION: This method for culturing Cordyceps militaris comprises using dried chrysalis (preferably dried silkworm chrysalis) as a culturing substrate, sterilizing the culturing substrate by a method for adding city water in an amount of 100ml based on 100g culturing substrate to the culturing substrate, passing steam through the substrate at 120 deg.C for 20min, then inoculating the sterilized culturing substrate with Cordyceps sinensis Sacc. (preferably Cordyceps militaris) and culturing Cordyceps sinensis Sacc. to form a mycelium, successively embedding the mycelium into soil, preferably vermiculite-like soil, culturing the mycelium so that the fruit body germinates on the soil surface. Furthermore, culture of Cordyceps sinensis Sacc. in culturing substrate is at first carried out under dark conditions at 20 deg.C for 30 days and then, after the mycelium is formed over whole culture substrate, the culture is preferably carried out at 400-600 lux for 30-50 days until the mycelium exhibits red color tone. |
